Gene delivery to a potential transgenic plant
Various methods of gene delivery to a potential transgenic plant. The uptake of foreign DNA or transgenes by plant cell is called transformation. A variety of techniques have been used to introduce transgene into plant cell. This can be grouped into following two major categories:- a) Agrobacterium mediated gene transfer. b) Direct gene transfer. a) Agrobacterium mediated gene transfer :- Agrobacterium tumefaciens is a remarkable species of soil-dwelling bacteria that has the ability to infect plant cells with a piece of its DNA. When the bacterial DNA is integrated into a plant chromosome, it effectively hijacks the plant’s cellular machinery and uses it to ensure the proliferation of the bacterial population. The gene transfer through Agrobact...