Practical: Study of Nostoc

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 Practical: Study of Nostoc Nostoc is a filamentous, photosynthetic cyanobacterium. It commonly occurs in freshwater, moist soil, wet rocks and other damp habitats. Colonies are usually gelatinous because numerous filaments remain embedded in a mucilaginous matrix. Materials Required Fresh or preserved Nostoc material, clean glass slide, cover slip, dropper, dissecting needle, water, blotting paper and compound microscope. Procedure Take a small quantity of Nostoc colony with the help of a dissecting needle and place it on a clean glass slide. Add one or two drops of water and gently tease the material so that the filaments become separated. Place a cover slip carefully over the material without trapping air bubbles. Remove excess water with blotting paper. First observe the preparation under low power and then under high power of the compound microscope. Observations The plant body of Nostoc is thalloid and usually forms a soft, jelly-like colony. A colony contains numerous ...

Ascobolus Character

Sample F1

Characteristics:

 1.  The thallus is made of richly branched mycelium that forms a complex structure and finally a cup shaped structure.

2. The hyphal masses penetrate the substratum. These act as organs of absorption for the aerial branches.

3. The hyphae are branched and septate. Each cell is multinucleate.

4. The male reproductive organs are antheridia and the female reproductive organs are called ascogonia.

5. Antheridia and ascogonia are borne at the tips of separate branches.

6. Antheridium is borne at the tip of antheridial branch. It is cylindrical or clavate in shape.

7. Each antheridium is multinucleate.

8. Ascogonium is also present at the tip of the ascogonial branch. It is sub-globose in shape and is multinucleate.

9. The ascocarps are apothecia and develop as a result of fertilization.

10. These are yellowish and saucer shaped.

11. Apothecium is a cup-shaped structure that is made of mycelium.

12. The section shows three zones-the outermost called hymenium, the middle called subhymenium and the lowermost called hypothecium.

13. The lowermost hypothecium, is made of sterile hyphae, loosely packed to form pseudoparenchymatous region called trama.

14. The middle zone consist of a few erect hyphae.This later merges with hymenial layer which is called sub-hymenium.

15. The hymenium consists of asci intermingled with paraphyses.

16. The asci elongate on maturation to protrude above the hymenial surface. These are found mixed with paraphyses. Ascus is a long and cylindrical structure. It opens by a terminal pore called operculum.

17. Each ascus contains eight ascospores. Ascospore is one celled, large in diameter, purple or dark brown and the spore wall shows longitudinal colourless striations.

.........................................................Hence it is Ascobolus.




Systematic Position:

Kingdom-Mycota

Division-Eumycota

Sub-division-Ascomycotina

Class-Discomycetes

Order-Pezizales

Family-Pezizaceae

Genus-  Ascobolus






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