Aceto Orcein Stained Onion Root

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Endoenzymes and Exoenzymes
Enzymes that catalyze biochemical
reactions in cells in which they were produced are called endoenzymes or
intracellular enzyme. Most of the enzymes involving in the primary and
secondary metabolisms of plants are endoenzymes. These enzymes never come out of
the cells under normal conditions until the cell wall and plasma membrane
are disturbed. After completion of the lifespan, they are destroyed within
the cell in which they were produced. Enzymes that catalyze biochemical
reactions outside the cells in which they were produced are called
exoenzymes or extracellular enzymes. Desmolysing enzymes that breakdown the
complex materials outside the cells are all exoenzymes. They are usually
secreted by heterotrophic microbes such as bacteria and fungi. Proteases,
cellulases, amylases, lipases, etc. are examples for exoenzymes. These
enzymes come out of the cell and do catalytic reactions in the cell's
environment. Examples-Exoenzymes are usually produced by microbes to hydrolyze
insoluble complex food materials in the medium or habitat. Insectivorous
plants secrete proteolytic enzymes through its glands on leaves to digest
proteins in the prey. In the grains of cereals, the cells of scutellum and
aleurone layer secrete hydrolytic enzymes into endosperms to dissolve the
stored food materials which are to be made available to germinating
seedling.
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