Practical: Study of Nostoc

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 Practical: Study of Nostoc Nostoc is a filamentous, photosynthetic cyanobacterium. It commonly occurs in freshwater, moist soil, wet rocks and other damp habitats. Colonies are usually gelatinous because numerous filaments remain embedded in a mucilaginous matrix. Materials Required Fresh or preserved Nostoc material, clean glass slide, cover slip, dropper, dissecting needle, water, blotting paper and compound microscope. Procedure Take a small quantity of Nostoc colony with the help of a dissecting needle and place it on a clean glass slide. Add one or two drops of water and gently tease the material so that the filaments become separated. Place a cover slip carefully over the material without trapping air bubbles. Remove excess water with blotting paper. First observe the preparation under low power and then under high power of the compound microscope. Observations The plant body of Nostoc is thalloid and usually forms a soft, jelly-like colony. A colony contains numerous un...

Comparison of Internal Structure of Stem of Selaginella and Lycopodium

 

Comparison of Internal Structure of Stem of Selaginella and Lycopodium


Characteristics

Selaginella Stem

Lycopodium Stem

Plant group

Heterosporous lycophyte

Homosporous lycophyte

Stem nature

Usually delicate and branched

Usually firm and dichotomously branched

Epidermis

Single-layered epidermis with cuticle

Single-layered epidermis with cuticle

Stomata on stem

May be present in young stem

Usually few or absent on stem

Cortex

Well-developed cortex; often differentiated into outer sclerenchymatous and inner parenchymatous regions

Broad cortex; commonly differentiated into outer, middle, and inner regions

Outer cortex

Generally sclerenchymatous and provides mechanical support

Often sclerenchymatous or compact parenchymatous

Inner cortex

Usually parenchymatous

May be parenchymatous; sometimes thick-walled

Air spaces

Large air spaces may be present around the stele

Air spaces are generally absent

Endodermis

Present as special elongated cells called trabeculae

May be present, but not in the form of trabeculae

Trabeculae

Present; they suspend the stele in the air cavity

Absent

Pericycle

Present around vascular tissue

Present around vascular tissue

Stele type

Mostly protostele; may be monostelic or polystelic

Mostly protostele; commonly actinostele, plectostele, or mixed protostele

Number of steles

One or more steles may occur

Usually single stele

Xylem arrangement

Xylem is generally central and exarch

Xylem is central, exarch, and may be star-shaped or plate-like

Protoxylem position

Protoxylem is towards the outside

Protoxylem is towards the outside

Metaxylem position

Metaxylem is towards the center

Metaxylem is towards the center

Phloem position

Phloem surrounds or lies between xylem regions

Phloem occurs between xylem arms or plates

Pith

Usually absent

Usually absent

Vascular complexity

Stele may be suspended by trabeculae, giving a specialized appearance

Vascular cylinder is compact and not suspended

Special feature

Presence of trabeculae and air cavity around stele

Variety of protostele types, especially plectostele in many species

Main difference

Stele is often suspended in air spaces by trabeculae

Stele is compact, without trabeculae




Major difference: Selaginella stem is recognized by trabeculae and air spaces around the stele, whereas Lycopodium stem has a compact protostele without trabeculae.

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