MCQ BOTANY PRACTICE SET 24.04.2020

1. In electron microscopy, to detect specific macromolecule or structure such as spindle pole body

(SPB), the frequently used procedure is to couple secondary antibody with

(a) Alexa 568 

(b) Cy5

(c) Gold particle 

(d) Osmium tetraoxide

2.Which of the following statements is correct for the process of speciation?

(a) Allopatric speciation occurs between adjacent populations.

(b) Parapatric speciation may, occur between geographically separated populations.

(c) Sympatric speciation occurs within one continuously distributed population.

(d) Sympatric speciation occurs when continuously distributed populations are fragmented.

3.Which one of the following does not contribute to microevolutionary change?

(a) Mutation 

(b) Random mating

(c) Genetic drift 

(d) Natural selection

4. A multimeric proteins when run on an SDS gel showed 2 bands at 20 kDa and 40 kDa. However, when the protein was run on a native gel, showed a single band at 120 kDa. The native from of the protein would be

(a) homotrimer 

(b) heterotetramer

(c) heterodimer 

(d) heterotimer

5. E. coli mutants isolated from a genetic screen showed following classes of mutations

(A) Points mutations in lacl

(B) Deletions immediately downstream of the transcription start site of the lacZYZ mRNA

(C) Duplication of part or whole of lacY

(D) Duplication of part of whole of Lac A

Choose the option which is likely to result in constitutive expression of the lac operon?

(a) Both (A) and (B) 

(b) Both (B) and (C)

(c) Both (C) and (D) 

(d) Only (A)

6.An integral membrane protein (P) has been identified as a cell surface protein of hepatocytes and assigned to bind to hepatitis B virus (HBV) and promote its entry into cytosol. Upon binding to HBV particles, the C-terminal of P interacts with F-actin in the cytosol and in turn, helps in the entry of the HBV particles. P was successfully cloned and expressed in animal cells in culture where in its N-terminal is exposed on the surface while the C-terminal resides in the cytosol. The recombinant protein P so expressed retains its complete structure and function. From the list of experiments given below, which one of the experiments will you perform to show the C-terminal of the protein P via interacting with F-actin helps in HBV entry ?

(1) Incubating radiolabelled HBV with hepatocytes in culture and follow up its association with F-actin by immuno-precipitation analysis using anti-F-actin antibody.

(2) Incubating radiolabelled HBV with hepatocytes over-expressing the C-terminal mutant of P and repeat the rest of the experiment as in "I".

(3) Incubating radiolabelled HBV with hepatocytes over expressing the N-terminal mutant of

P and repeat the rest of the experiment as in "I".

(4) Using wild type P as well as C-terminal mutant of P and their individual over expression

in a heterologous cell line (completely devoid of endogenous P protein) and then repeat

experiment as in "I".

7. Which of the following statements are correct?

(i) Dikaryon in higher fungi is n + n nuclei containing, where two nuclei are commonly different in genetic makeup.

(ii) Fungus growing on dung is coprophilous, where as growing on bark is corticolous.

(iii) Disease of fungus producing ‘sooty mass’ of spores is called smut, which is caused by Puccinia.

(iv) Alternaria, Colletotrichum and Trichoderma belong to Class Deuteromycetes.

(v) Morchella is an edible mushroom of Class Basidiomycetes.

(a) (i), (ii) and (iv)

(b) (ii), (iv) and (v)

(c) (i), (ii), (iv) and (v)

(d) All the five statements are true.

8.Which of the following environmental conditions essential for the optimum growth of Rhizopus on a stale bread piece?

(i) Darkness (ii) Relative humidity 95%

(iii) Well lighted place

(iv) Temperature 5°C

(v) Relative humidity about 10%

(vi) Temperature of about 25°C

(a) (i), (ii) and (vi) 

(b) (ii), (iii) and (vi)

(c) (i), (v) and (vi) 

(d) (i), (iv) and (v)

9.Which of the following criteria used for classification of Fungi into its classes?

(a) Mode of spore formation

(b) Morphology of mycelium

(c) Fruiting bodies

(d) All of these

10.Assume that a fern sporophyte has two fertile leaves. Each containing 10 fertile pinnae. Each fertile pinna contains 4 sori. If each sorus contains 5 sporangia and each sporangium contains 6 spore mother cells, then the total number of spores produced will be

(a) 9600 

(b) 2400

(c) 4800 

(d) 19,200

11. Leaves of conifers are well adapted to withstand extremes of temperature, humidity and wind, because of the following feature/s.

(a) Acicular leaves 

(b) Absence of venation

(c) Sunken stomata 

(d) All of these

12. Which of the following is a true statement?

(a) Ferns are the first to appear after forest fire.

(b) Chilgoza nuts are fruits of Pinus gerardiana.

(c) Ovuliferous scale is equivalent to stamen.

(d) Coralloid roots are associated with Anabaena for N2 fixation in Cycas, which are positively geotropic.

13.Bryophytes are different from Fungi in having

(a) terrestrial habitat

(b) sterile jacket around sex organs

(c) multiflagellate gametes

(d) gametophytic body

14. Deep in the tropical rain forest, a botanist discovered an unusual plant with vascular tissues, stomata, a cuticle, flagellated sperm, strobilus like reproductive structures bearing seeds and an alternation-of-generations in the life cycle. He was very excited about this discovery because it would be rather unusual for a plant to have both

(a) a strobilus and flagellated sperm

(b) vascular tissues and alternation of generations

(c) seeds and flagellated sperm

(d) alternation of generations and seeds.

15.In which one pair both the plants can be vegetatively propagated by leaf species?

(a) Agave and Kalanchoe

(b) Bryophyllum and Kalanchoe

(c) Asparagus and Bryophyllum

(d) Chrysanthemum and Agave

16.Which of the following is true statement?

(a) Pribnow box lies in mRNA in upstream stream sequence.

(b) In clover leaf model of tRNA, amino acid is attached to 5′ by ester bond, if it is a charged one.

(c) Translation is initiated by s factor, formed of γ-chain, of RNA polymerase enzyme.

(d) Sn RNA, 5S RNA and tRNA are transcribed with the help of RNA polymerase III in eukaryotes.

17. In bacteria, the site for respiration is

(a) episome 

(b) mesosome

(c) cytoplasm 

(d) plasmid.

18. Bacterial flagella are mainly made up of

(a) protein 

(b) amines

(c) lipids 

(d) carbohydrates.

19. Cyanobacteria are

(a) cyanophycean members which infect bacteria

(b) virus which infect blue green algae

(c) autotrophic prokaryotes with characteristic blue green pigments

(d) bacteria which infect cyanophycean algae.

20. Monerans that are devoid of cell wall are

(a) actinomycetes 

(b) cyanobacteria

(c) mycoplasma 

(d) eubacteria.

21. Some hyperthermophilic organisms that grow in highly acidic

habitats belong to the two groups

(a) eubacteria and archaea

(b) cyanobacteria and diatoms

(c) protists and mosses

(d) liverworts and yeasts.

22.DNA base sequence is polymeric if

(a) it is present in more than 1000 genes

(b) a variant sequence exist in at least 1% of population

(c) it can’t be cut by exonuclease and endonuclease enzyme

(d) the carrier frequency is between 2-8%.

23. Vegetative reproduction by layering is found in

(a) rose 

(b) jasmine

(c) mango 

(d) all of these

24.The best source of Vitamin C among the following:

(a) Lycopersicum esculentum                  (b) Cirus medica

(c) Capsicum annum                                (d) Phyllanthus emblica

25. The growth phase of an organism before attaining sexual

maturity is referred to as

(a) juvenile phase 

(b) vegetative phase

(c) both (a) and (b) 

(d) none of these