MCQ ABOUT CENTRAL DOGMA
1. The amount of which of these is least in a cell
(a) mRNA (b) tRNA (c) rRNA (d) None of these
2.Who among the following established that RNA is the genetic material?
(a) Lederberg (b) Griffith
(c) Nirenberg and Holley (d) Frankel Conrat
3. The ratio of DNA in bacterial cell and eukaryotic is about
(a) 1: 100 (b) 1: 1000 (c1: 10000 (d) 1: 1 lakh
4. The number of nucleotides constituting tRNA is usually
(a) 40 (b) 80 (c) 120 (d) 160
5. Most stable RNA is
(a) mRNA (b) tRNA (c) rRNA (d) sRNA
6. L-shaped model of tRNA was proposed by
(a) Hoagland (b) Holley (c) Klug (d) Kornberg
7. Aminoacyl synthetase binding loop in tRNA is
(a) ‘PI’C loop (b) DHU loop (c) anticodon loop (d) extra arm
8.The RNA that picks up specific - amino- acids from the amino acid pool in the cytoplasm and bring them to ribosome during protein synthesis is called
(a) m- RNA (b) t- RNA (c) r -RNA (d) carrier RNA
9. The scientist who first synthesized DNA in vitro was
(a) A Kornberg (b) A Garrod
(c) J D Watson (d) H G Khorana
10.In Rous sarcoma virus, the flow of information is
(a) DNA ®RNA®Protein
(b) DNA ® Protein ® RNA
(c) RNA® DNA® RNA® Protein
(d) RNA ® DNA® Protein
11.RNA and DNA were artificially synthesized by
(a) Ochoa and Nirenberg (b) Ochoa and Kornberg
(c) Kornberg and Nirenberg (d) Nirenberg and Khorana
12. Amino acid binding site in tRNA is
(a) anticodon loop (b) 5/end (c) DHU loop (d) CCA 3/ end
13. The informations are carried from gene to cytoplasm by
(a) mRNA (b) rRNA (c) tRNA (d) None of these
14. The following is needed during DNA replication
(a) DNA polymerase and DNA ligase
(b) ENA polymerase and translocase
(c) DNA polymerase only
(d) DNA ligase only
15.The enzyme topoisomerase is helpful in
(a) unwinding of the helix (b) breaking of DNA strand
(c) proof reading of replicatingDNA (d) synthesis of primer
16. DNA polymerase-I is involved in
(a) formation of RNA primer (b) filling of gap
(c) joining of
17. In eukaryotes, DNA synthesis on leading strand is catalysed by
(a) DNA polymerase a (b) DNA polymerase b
(c) DNA polymerase d (d) DNA polymerase s
18. DNA generally acts as a template for the synthesis of
(a) only protein (b) only DNA (c) only RNA (d) Both (b) and(c)
19.RNA retroviruses have special enzyme that
(a) polymeries host DNA (b) translates host DNA
(c) disintegrates host DNA (d) transcribes viral RNA to cDNA
20. Ligase enzyme is used for
(a) denaturation of DNA (b) splitting of DNA into small bits
(c) joining bits of DNA (d) digestion of lipids
21. DNA polymerase enzyme was discovered by
(a) Kornberg (b) Nirenberg (c) Ochoa (d) Watson
22.Who discovered transcription?
(a) Beadle and Tatum (b) Temin and Baltimore
(c) Watson and Crick (d) Khorana reverse
23The mRNA binds to the small subunit of ribosomes in the presence of initiation factors which are
(a) monosaccharides (b) polysaccharides
(c) lipids (d) proteins
24. During protein synthesis, amino acid gets attached to t-RNA with the help of
(a) mRNA (b) aminoacyl synthetase (c) ribosome (d) rRNA
25. In E. coil,
(a) initiation of RNA transcription of specific sites
(b) initiation of RNA transcription at all sites
(c) termination of RNA transcription at some sites
(d) termination of RNA transcription at all sites
26. Molecular weight of r - factor is
(a) 109000 (b) 5500 (c) 55000 (d) 10000
27. Find the incorrect match
(a) Core enzyme—α2ββ/w (b) Catalytic centre —β1β’
(c) Pribnow box—4 bp recognition site (d) p (
28.The core enzyme requires a factor for termination of RNA synthesis at some sites. This is known as
(a) sigma factor (b)
29.Cap nucleotides at 5’ of mRNA consists of
(a) m7G (b) m5C (c) poly A (d) CCA
30. In eukaryotes, RNAP - III catalyses the synthesis of
(a) All rRNA and tRNA (b) mRNA, HnRNA and SURNA
(c) 5S rRNA, tRNA and ScRNA (d) 28S, 18S and 5S rRNA
31.121. snRNA takes part in
(a) processing of polypeptide (b) RNA-splicing
(c) gene splicing (d) replication
32.The functional unit in the protein synthesis is
(a) peroxisome (b) dictyosome
(c) lysosome (d) polysome
33. In eukaryotes, initiation of polypeptide chain in protein synthesis is induced by
(a) methionine (b) glycine (c) leucine (d) lysine
34.The formation of polyribosomes from ribosomes is done in the presence of
(a) Na+ ions (b) K+ ions (c) Ca2+ ions (d) Mg2+ ions
35.The translation step in the process of protein synthesis, in which the conversion of the language of nucleic acid into that of proteins, is made by a charged form of
(a) mRNA (b) tRNA (c) rRNA (d) template DNA
36.Which of the following elongation factor has translocase activity?
(a) EF-Tu (b) EF-Ts (c) EF-G (d) All of these
37. Which factor is necessary to separate the both ribosomal subunits in E. coli translation process
(a) EF (b) IF (c) RF (d) TF
38 Protein synthesis in an animal cell takes place
(a) only on ribosomes present in cytosol
(b) on ribosomes present in nucleolus and cytosol
(c) on ribosomes present in cytosol and in mitochondria
(d) only on ribosomes attached to nuclear envelope and endoplasmic reticulum
39.. The first amino acid in any polypeptide chain of prokaryote is always
(a) formylated methionine (b) formylated arginine
(c) lysine (d) methionine
40.An antibiotic which inhibits translation in eukaryotes is
(a) chloromycetin (b) penicillin (c) puromycin (d) tetracycline
.jpg)
Comments
Post a Comment